sigX [2018-12-07 16:39:24]

RNA polymerase ECF-type sigma factor SigX, required for resistance against cationic antimicrobial peptides

Locus: BSU23100

Isoelectric point: 6.09

Molecular weight: 23.03 kDa

Protein length: 194 aa

Gene length: 582 bp

Function: resistance to cationic antimcrobial peptides

Product: RNA polymerase ECF-type sigma factor SigX

Essential: no

Synonyms: ypuM

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → Transcription → Sigma factors
• Information processing → Regulation of gene expression → Sigma factors and their control → Sigma factors
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,414,627 → 2,415,211

The protein

Protein family

• ECF subfamily (according to Swiss-Prot)

Localization

• cell membrane (according to Swiss-Prot)

Additional information

• Expression of the SigX regulon in increased in ugtP mutants PubMed

Expression and Regulation

Operons

• Genes: sigX-rsiX
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigX: sigma factor, PubMed, in SigX regulon

  Regulatory mechanism

  • YvrHb: activation, PubMed, in YvrHb regulon

  Regulation

  • induced by glucose PubMed, this depends on YlxR PubMed

Biological materials

Mutant

• MGNA-A179 (sigX::erm), available at the NBRP B. subtilis, Japan
• 1A901 ( sigX::spec), [Pubmed| ], available at BGSC
• BKE23100 (ΔsigX::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGAAACCCCTCCGTTC, downstream forward: _UP4_GAGCTTTTGAGGGAGGAGCT
• BKK23100 (ΔsigX::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGAAACCCCTCCGTTC, downstream forward: _UP4_GAGCTTTTGAGGGAGGAGCT

References

Reviews

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The SigX regulon

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Other original publications

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